共同實驗室(五)

組長   鄭仁坤 醫師

研究人員

楊道翔 博士、Raju Poongodi 博士、朱翠玉

進駐研究醫師：黃健中 醫師、王資俊 醫師

1. Establish animal pain (postoperative, inflammatory and neuropathic) models and explore the pathogenesis of different pain status.

2. Test the antinociceptive and neurotoxic effects of intrathecal analgesics.

3. We investigated the effects of extracellular visicles (EVs) derived from human umbilical cord mesenchymal stem cell (UCMSC) in animal models of nerve injury–induced pain and spinal cord injury.

陽明交通大學材料科學與工程學系：林欣杰 教授
馬偕醫學大學：彭賢佑 教授
馬偕醫院病理科: 王道遠 教授
馬偕醫研部：楊崑德 教授
馬偕醫院麻醉部主治、住院醫師參與研究計劃及實驗技術研習

1. 大鼠脊髓腔導管置入及持續給藥幫浦
2. 大鼠術後疼痛、癌症疼痛、神經受損後慢性神經痛、發炎性疼痛模型, spinal cord injury model
3. 大鼠熱痛覺、觸痛覺行為檢測、gait analysis
4. 西方墨點法、雙螢光免疫組織染色

In the past few years, we have explored the possible working mechanism and neurotoxic effect of intrathecal gabapentin, a calcium channel and NMDA-R modulator, in animal pain models. In addition, we found the antinociceptive effect of JNK inhibitor in the mice cancer pain model. We focused on the study of T-type calcium channels and cannabinoid receptors in the L5/6 spinal nerve ligation (SNL)-induced neuropathic pain. We presented several lines of evidence to show that CB1R and CB2R play important roles in modulating nerve injury-induced pain and neuroinflammation. Recently, We have explored the new therapy approach for neuropathic pain by developing a cell-free treatment that is able to retain the benefits of mesenchymal stem cell (MSC)-based therapy without the inherent difficulties of MSC. Umbilical cord MSC EVs display excellent and long-lasting antinociceptive effects in the SNL pain model. Furthermore, the homing ability and potential regenerative properties of UCMSC EVs make themselves a promising therapeutic candidate for nerve injury-induce pain. Recently, we also found the therapeutic effects of HucMSC-EV in the spinal cord injury model. Our works have been published in international high-impact factor scientific journals.

Poongodi R, Hsu YW, Yang TH, Huang YH, Yang KD, Lin HC, Cheng JK. Stem cell-derived extracellular vesicle-mediated therapeutic signaling in spinal cord injury. Int J Mol Sci. 2025 Jan 16;26(2):723. (BIOCHEMISTRY & MOLECULAR BIOLOGY) (IF: 4.9, Ranking: 72/320=22.5%)

Poongodi R, Yang TH, Huang YH, Yang KD, Chen HZ, Chu TY, Wang TY, Lin HC, Cheng JK. Stem cell exosome-loaded Gelfoam improves locomotor dysfunction and neuropathic pain in a rat model of spinal cord injury. Stem Cell Res Ther. 2024 May 20;15(1):143. (CELL & TISSUE ENGINEERING) (IF: 7.9, Ranking: 3/31=9.6%)

Lin YL, Yang ZS, Wong WY, Lin SC, Wang SJ, Chen SP, Cheng JK, Lu H, Lien CC. Cellular mechanisms underlying central sensitization in a mouse model of chronic muscle pain. Elife. 2022 Nov 15;11:e78610. (BIOLOGY) (IF: 8.713, Ranking: 8/94=8.5%)

Poongodi R, Chen YL, Yang TH, Huang YH, Yang KD, Lin HC, Cheng JK. Bio-scaffolds as cell or exosome carriers for nerve injury repair. Int J Mol Sci. 2021 Dec 12;22(24):13347. (BIOCHEMISTRY & MOLECULAR BIOLOGY) (IF: 6.208, Ranking: 69/297=23.2%)

Hsu JM, Shiue SJ, Yang Hsu JM, Shiue SJ, Yang KD, Shiue HS, Hung YW, Pannuru P, Poongodi R, Lin HY, Cheng JK. Locally applied stem cell exosome-scaffold attenuates nerve injury-induced pain in rats. J Pain Res. 2020 Dec 4;13:3257-3268. (CLINICAL NEUROLOGY) (IF: 2.832, Ranking: 139/212=65.6%)

Shiue SJ, Rau RH, Shiue HS, Hung YW, Li ZX, Yang KD, Cheng JK. Mesenchymal stem cell exosomes as a cell-free therapy for nerve injury-induced pain in rats. Pain. 2019 Jan;160(1):210-223. (CLINICAL NEUROLOGY) (IF: 7.926, Ranking: 21/212=9.9%)